Susceptibility of Ozonated Water Against P. Endodontalis and P. Gingivalis and S. Mutans an Invitro Study

Abstract

Background:Ozone is a very unstable and extremely oxidizing compound, that quickly returns to nascent oxygen molecule, and has been in use as an antibacterial, antifungal, antioxidant, anticancer, and antiviral agent. Due to these antimicrobial properties, ozonated water has also been in dentistry as a disinfectant. Furthermore, studies also revealed that aqueous ozone has better biocompatibility when compared with chlorhexidine, sodium hypochlorite, and hydrogen peroxide considering its antibacterial properties the objective of our study was to evaluate the susceptibility of ozonated water against Porphyromonasendodontalis, Porphyromonasgingivalis, and Streptococcus mutans.

Methodology: It was an in-vitro preclinical experimental study conducted at the dental OPD of a tertiary care hospital in Karachi.The samples of the organisms were collected from the microbiology lab and the organismsS. mutant,P. gingivalis and, P. endodontalis were grown in their appropriate media for further experiment.The ozonated water 1µl was used to wet the paper disc and placed in the organism’s growth plates. And sensitivity analysis was performed.

Results: The growth of colonies was calculated on growth media plates in samples. The comparison of samples showed a significant (p-value <0.05) decrease in number of colonies in ozonated water growth plates when compared to control plates. The sensitivity analysis showed that the most of the strains of S. mutant,P. gingivalis and, P. endodontaliswere sensitive to ozonated water (p=0.002)

Conclusion: The ozonated water reduced the number of colonies on agar plates and it showed sensitivity towards S. mutant,P. gingivalis and, P. endodontalis.