Assessment of Antimicrobial Effects of Thymus Vulgaris Leaves in Extracts of Methnol, Ethanol and N-Hexane against Pathogenic Bacterial Species
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Abstract
Antibacterial activity of Thymus vulgaris leaves was scrutinized in solvent i.e., methanol, ethanol and n-hexane contrary to Staphylococcus aureus, Proteus vulgaris, Salmonella typhi, E.coli and Klebsiellapneumoniaeover well diffusion method. Tremendous inhibitory effects of methanolic extract weredisplayed against E. coli and Proteus vulgaris(90% and 80% respectively). The extract on the other hand exhibited a good inhibitory effect against Klebsiellapneumoniae,Salmonella typhi and Staphylococcus aureus (72%, 64% and 60%correspondingly). Moreover, the inhibitory effect of ethanolic extract showed also a worth noticing results against Proteus vulgaris,E. coli (72% and 70%) and showed adequate result against Salmonella typhi, Klebsiellapneumoniaeand Staphylococcus aureus (56%, 54% and 43% respectively).Furthermore, n-hexanoic extract disclosed good results against E. coli, Klebsiella pneumonia and showed average results contrary toSalmonella typhi, Staphylococcus aureus and Proteus vulgaris (65%, 63%, 52% and 36%).The E. coli was determined for the minimum inhibitory concentration.Precisely the MIC results were attained with Thymus vulgaris extract against E. coli. Results were affirmative for the inhibiting effects of the extracts of Thymus vulgaris against numerous bacterial species entailing its use as a resilient antibacterial agent providing a scientific ground for the advance research. the increased resistance of microorganisms to conventional chemicals and drugs is a serious and obvious worldwide problem, and research has been urged to identify new biocides with good activity. The purpose of this work is to replace synthetic antibiotics with natural plant extract of Thymus vulgaris, a drug that replaces commercially available antibiotics.