Anticoagulant and Thrombolytic Activities of Leaves Extract of Mangiferara Indica

Abstract

In this study anticoagulant and thrombolytic activities of Mangifera indica have been investigated which were not present in literature because ethnobotanical evidences have not been done before. The thrombolytic and anticoagulant (in vitro and in vivo) activities also phytochemical screening and high performance liquid chromatography of aqueous-methanolic (70:30) leaves’ extract of M. indica have been investigated. For in vitro experiment M. indica displayed a noteworthy (p<0.05) increment in prothrombin time, clotting time and activated partial thromboplastin time while in vivo experiment noteworthy (p<0.05) increase in clotting time, bleeding time, activated partial thromboplastin time and prothrombin time in a dose-dependent manner in rabbits after one week of treatment while heparin being taken as a positive control. For in vitro experiment, aqueous-methanolic extract in a dose-dependent manner displayed noteworthy (p<0.05) clot lysis while streptokinase being taken as a positive control. Moreover, HPLC showed the presence of mangiferin, quercetin and isoquercetin which down regulate the activity of factor Xa as well as act on antithrombin and plasminogen respectively, in coagulation cascade.