Propofol Inhibits Lung Cancer Invasion and Metastasis by Attenuating Icam-1/ Mmp-9 Axis

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Xin Huang, Guangbin Liang, Jian Mo, Weiming Liang, Fengmin Ge, Haiyan He, Daheng Li

Abstract

To investigate the effect of propofol on invasion and metastasis of A549 cells and the expression of intercellular adhesion molecule 1 (ICAM-1) / matrix metalloproteinase-9 (MMP-9) axis.Methods: A549 cells were treated with gradient doses of propofol (25, 50, 100 μg/ml) and different times (12, 24 h). The influences of propofol on ICAM-1 mRNA of A549 cells were observed by qRT-PCR, and the roles of different concentrations of propofol on ICAM-1 and MMP-9 protein and invasion ability were detected by Western Blot and Transwell assay, respectively.Results: The maximum inhibitory influence of propofol was achieved in group 100 μg/ml for 24 h. ICAM-1 and MMP-9 protein in the treatment group with 25, 50 and 100 μg/ml propofol for 24 h were significantly lower than those in the control group. The number of migrated and invaded cells in the 25, 50 and 100 μg/ml propofol treatment group for 24 h was significantly lower than that in the blank group.Conclusion: ICAM-1 protein and MMP-9 protein in human lung cancer A549 cells could be down-regulated after treatment with 50 and 100 μg/ml propofol, and the invasion and metastasis ability of A549 cells could be attenuated through ICAM-1/ MMP-9 axis.

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