Effect of Yiqi Huoxue Huatan Tongluo Method on Intestinal Flora Expression in Patients with Acute Cerebral Infarction

Abstract

To evaluate the clinical effect of Yiqi Huoxue Huatan Recipe (recipient for invigorating qi, activating blood and resolving phlegm) on ischemic stroke patients and its effect on intestinal flora expression through clinical study of Yiqi Huoxue Huatan Recipe, and to explore its possible mechanism and provide clinical basis for its further application. Methods: 60 patients with acute cerebral infarction were divided into treatment group (30 cases, combined Chinese traditional and Western medicine) and control group (30 cases, pure Western medicine). Fecal samples were collected at 24 hours and 14 days after the onset of the disease, and 16SrDNA high-throughput sequencing was used to observe the status of the faecal samples and the changes of intestinal flora abundance. To compare whether there was a difference between the two groups at 24 hours and 14 days after the onset of the disease. To compare whether there is a difference in microbiological status between the two groups during the same time period. To compare the difference of TCM syndrome integral between the two groups. Results: Before and after treatment of the patients with cerebral infarction in the two groups, those ranked the top six at the level of intestinal flora are: Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Tenericutes and Cyanophyta. After treatment, the abundance of Bacteroides was up-regulated, while the abundance of Firmicutes and Proteobacteria was down-regulated. However, the adjustment range of treatment group with combined Chinese medicine and western medicine was larger and there was significant difference between the two groups (P<0.05). The improvement of TCM syndrome in the treatment group was more significant than that in the control group (P<0.05). Conclusion: Intestinal flora disturbance may be a potential causative factor of cerebral ischemic diseases. Yiqi Huoxue Huatan Recipe has a certain effect on the abundance of intestinal flora, and the symptom of the patients in the treatment group is improved more obviously. By regulating the intestinal flora, the patients' internal environment is improved and the constitution is improved, which may be one of the effective mechanisms of Yiqi Huoxue Huatan Recipe.