Resveratrol affects Proliferation, Migration, Invasion and Apoptosis of Oral Squamous Cell Carcinoma Cells by Inhibiting the WNTPathway

Abstract

To explore the mechanism by which resveratrol (RES) affects the proliferation, migration, invasion and apoptosis of oral squamous cell carcinoma (OSCC) cells through inhibiting the Wnt pathway.

Methods: Ki-67, matrix metalloproteinase-9 (MMP-9) and B-cell lymphoma-2 (Bcl-2)protein expression levels were detectedbyimmunohistochemical staining. Tca8113 cells were treated with different concentrations of RES (0, 50, 100 and 200 μmol/L) and dividedinto control, low-dose RES, medium-dose RESand high-dose RES groups. EdU staining, Transwell migration and invasion assays and AnnexinV-FITC/PI double-stainingwere employed to determinecell proliferation, migration, invasion and apoptosis, respectively. E-cadherin and N-cadherinmRNA expression levels incells were analyzed by RT-qPCR.Wnt1, β-catenin and glycogen synthase kinase-3β (GSK-3β) protein expression levels were detected by Western blotting.

Results: Ki-67, MMP-9 and Bcl-2 were positively expressed in OSCC tissues, with higher rates than those in normal oral mucosal tissues. RES significantly inhibited the proliferation, migration and invasion ability of Tca8113 cells, down-regulated the expression levelsof N-cadherin mRNA and Wnt1, β-catenin and GSK-3β proteins, promoted apoptosis, and up-regulated E-cadherinmRNA expression leveldose-dependently(P<0.05).

Conclusion: RES can inhibit the proliferation, migration and invasion of OSCC cells and promote apoptosispossibly by inhibiting the Wnt signaling pathway, providing a potential target for targeted therapy of OSCC.