Bioinformatics Analysis of the Microarray Data of Broiler Chicken Spleen Vaccinated with ND Vaccine Genotype VII and Challenged with Clostridium Perfringens Type A

Abstract

Necrotic enteritis is one of the most deadliest illnesses that affect most species of birds including broiler that developed due to infection by pathogenic strain of Clostridium perfringens, most of its pathogenesis caused by NetB and TpeL toxins Therefore, the current study was designed to define most genes, microRNA and different molecular and biological pathways implicated in the pathogenesis of NE. Differentially expressed genes obtained from GEO database (GSE13085) by applying GEO2R analysis. ShinnyGo 8.0 software were applied to identify the set of possible diseases associated with the obtained DEGs and Protein-protein interaction (PPI) network obtained from String database and visualized by Cytoscape software. microRNAs that regulate most of DEGs obtained from Network Analyst. We found that top 5 upregulated hub genes were TLR-4, STAT-1, TRAF-6, Casp-8, and cxcl-12, top 3 downregulated hub genes were CD4, BCL-2, and CD44. Additionally toll like- receptor signaling pathway and cytokine- cytokine receptor interaction was the most implicated pathways NE. furthermore gga-mir-143 is microRNA that regulate most important DEGs. Based on our finding we can suggest that TLR-4, Casp-8, CD4, BCL-2, and gga-mir-143 represent a novel therapeutic target for NE induced immunosuppressive effect.