GC-MS analysis Antioxidant, Anti-diabetic study of Volatile alkaloids extract of Deverra scoparia (Coss. & Dur.) (Apiaceae)

Abstract

Background: Deverra scoparia Coss&Dur (Apiaceae) is an endemic Algerian plant known widely for its folk medicinal uses.

 Objectives: The present research aimed to evaluate the pharmacological properties and chemical constituents of the volatile alkaloid (VA) extract of D. scoparia.

 Methods: The aerial part of D. scoparia was hydro-distilled to obtain the VA extract, followed by fractionation based on different PH mediums, two fractions; diethylic ether (ETV) and dichloromethane (DV). Determination of the alkaloid amount (AA), the characterization analysis with GC-MS, and the in vitro pharmacological properties presented as an anti-oxidant and anti-diabetic assay for the ETV and DV fractions.

 Results: The DV showed a high  alkaloid amount (AA) concentration 62.89 ±0.05 µg EA/mg with nine (9) alkaloid compounds identified by Gas chromatography-mass spectroscopy GC-MS analysis, while the anti-oxidant potential was assessed via DPPH radical scavenging capacity and β-carotene linoleic acid bleaching assay, which revealed that ETV had a low IC50= 354.24± 2.20 and 67.60± 2.10 µg/ml respectively. Whereas, the anti-diabetic activity based on the inhibition of α-amylase enzyme the DV represented a remarkable inhibition IC50=163.69± 1.41µg/ml.

 Conclusions: Due to the works’ demonstration of the VA's significant pharmacological potential, future studies can concentrate on isolating alkaloid compounds and examining their pharmacological characteristics in vivo.