Antibacterial Activity of Chitosan-Based Materials
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Abstract
Chitosan is a naturally occurring polymer with powerful antimicrobial properties against bacteria, yeasts and molds. The present work consists in developing chitosan-based materials and also in examining their in-vitro antimicrobial activities on commensal and pathogenic bacterial strains that are most often associated to a great extent with the microbiological deterioration of food. The bactericidal activity of these materials was investigated against two Gram-negative bacteria, i.e. E.coli ATCC25022 and Pseudomonas, and two Gram-positive bacteria, i.e. Bacillus ATCC29522 and Staphlococcus ATCC29422. The agar well diffusion method was used for the antibacterial study.
The results found showed that Gram-positive bacteria were more sensitive than Gram-negative ones to the action of chitosan. In addition, it turned out that the clay/chitosan material showed a large inhibition zone diameter (Q = 41 mm) on the strain of Escherichia coli ATCC 25022.