Study of Biological Effectiveness of the Aqueous Extract of Date Pits( Phoenixdactylifera.L) Grows in the Algerian Oases

Abstract

The date palm pit (Phoenix dactylifera L.) is a byproduct of the date business that contains a number of bioactive compounds with potential health benefits. This study investigated the preparation of aqueous extract of date palm pit (DPP) and its biological activities, including antioxidant, anti-amylase, and antimicrobial activities.The total phenolic content of the DPP aqueous extract was 128.02 ± 0.35 µg GAE/mg gallic acid equivalent (GAE)/g extract. The total flavonoid content was 82.12 ± 0.89 µg QE/ mg /g extract.The DPP aqueous extract showed significant antioxidant activity in the DPPH free radical scavenging assay (IC50 = 492 ± 0.4 µg/mL), and the FRAP assay (RC50 = 408 ± 1.87 µg/mL).The DPP aqueous extract showed moderate anti-amylase activity (IC50 = 4.089 ± 0.78 μg/mL). The DPP aqueous extract also showed antimicrobial activity against all five bacterial strains and the yeast strain tested. The highest inhibitory zone diameter was observed against Klebsiellapneumoniae (20 mm).