Pretreatment Effect of Bisoprolol on the Serum Levels of ALP and LDH Enzymes in the Liver ischemia-reperfusion Model in Rats

Abstract

Introduction: Liver ischemia-reperfusion injury occurs primarily after liver surgeries and liver transplants. In addition to the liver, it can involve systemically different organs of the body. In some cases, this injury is irreversible and may result in multi-organ dysfunction and death. Some studies report that bisoprolol can reduce the injury caused by ischemia-reperfusion in the heart and some other tissues. The present study investigates the effect of bisoprolol as a pretreatment on the serum levels of ALP and LDH enzymes in the liver ischemia-reperfusion model in male rats.

Methods: In this experimental study, 28 Wistar rats were randomly divided into 4 groups of seven (n = 7). They included the sham group that received 2 ml Normal Saline (NS) daily through gavage for 7 consecutive days and then surgery without induction of ischemia-reperfusion (IR), the BSP group that received 10 mg/kg BSP (dissolved in 2 ml of normal saline) through gavage for 7 consecutive days and then surgery without IR induction, IR group that received 2 ml NS daily through gavage for 7 consecutive days and then surgery and IR induction, and IR + BSP group that received 10 mg/kg BSP through gavage for 7 consecutive days, then surgery and IR induction. After the desired time, blood was collected from the heart of the rats. Then, the rats were euthanized by an incision in the diaphragm. After clotting and centrifugation of the blood samples, the upper part was removed to measure the desired factors.

Results: The mean serum concentration of ALP (alkaline phosphatase) in the BSP group did not change significantly compared to the Sham group (P>0.05). Also, the mean serum ALP level in the IR + BSP group did not change significantly compared to the IR group (P>0.05). The mean serum concentration of LDH (lactate dehydrogenase) in the IR + BSP group compared to the IR group and the BSP compared to the Sham group did not change significantly (P>0.05).

Conclusion: Based on the results, pretreatment with bisoprolol drug before the start of liver ischemia-reperfusion with the mentioned dose and the conditions of this research probably cannot cause a significant change in the serum levels of ALP and LDH enzymes.