Angelica Sinensis Polysaccharide Mediates Sirt1 to Inhibit P53/P21 Pathway and Delay Hematopoietic Stem Cell Aging

Abstract

To study the effect of Angelica sinensis polysaccharide (ASP) on the expression of Sirt1/p53/p21 in radiation-induced aging hematopoietic stem cells (HSCs) of mice, and to explore the possible mechanism of ASP regulating the aging of HSCs. Methods: C57BL/6J mice were randomly divided into control group, model group and ASP group. The model group was irradiated with X-ray 3.0Gy/8F to establish the aging model of HSCs in mice. ASP group was given ASP intragastric administration during irradiation; The control group and the model group were given equal volume of normal saline. HSCs were sorted by immunomagnetic beads, and cell cycle was detected by flow cytometry The aging cells were observed by β-galactosidase staining. The directional differentiation ability of HSCs was observed by mixed colony culture (CFU-Mix). The expression of Sirt1, p53 and p21 protein was detected by Western blot. Results: Compared with the control group, X-ray can significantly increase the proportion of HSCs G1 cells, the rate of SA-β-Gal positive cells and the expression of p53 and p21 protein in aging group (P<0.05). The expression of Sirt1 and mixed colony forming ability were decreased (P<0.05). Compared with the model group, ASP inhibited the increase of aging HSCs G1cell ratio, SA-β-Gal positive cell rate and p53 and p21 protein expression (P<0.05). At the same time, Sirt1 expression and mixed colony forming ability were increased (P<0.05). conclusion: ASP may inhibit p53/p21 pathway and delay the aging of mouse HSCs by regulating Sirt1.