Prevalence and Antimicrobial Susceptibility Pattern of E.Coli Isolated From Raw Meat Samples in the Local Markets of Peshawar

Abstract

Food-borne pathogens are the leading cause of illness and death in developing countries costing billions of dollars in medical care and social costs. Contaminated raw meat is one of the main sources of food-borne illnesses. The present study was carried out to isolate, identify and the prevalence of E.coli from raw meat samples. Antibiotic susceptibility pattern of the isolated bacterial species. The study was conducted at Abasyn University, Peshawar. In the present study 50 raw meat samples were collected from local markets of Peshawar. Out of 50 samples 40 samples showed significant bacterial growth. The most common bacterial species isolated were E. coli and Salmonella spp. Among these identified isolates E.coli counted for 35 samples and Salmonella spp. counted for 5 samples. The prevalence of E coli was (87%) and Salmonella spp. was (13%). The susceptibility of E.coli and salmonella spp. isolates from meat samples against various antimicrobial agent (Cefixime, Ciprofloxacin, Cefoperazone, Ceftriaoxone, Moxifloxacin, Mermopene and Vancomycin) was determined by disc diffusion method. E.coli was sensitive mostly to Moxifloxacin and Salmonella spp. were found sensitive to Cefixime and mostly resistance towards Meropenem. The results have also established retail meats represent hazards to human health and can be a threat to public health. On account of this, it is necessary that the consumers adopt the basic instructions regarding good hygienic practices, adequate good cooking of meat, adequate storage temperature and cross-contamination.