Molecular Characterization of Penicillium Chrysogenum Isolated from Soil and Their Antibacterial Activity against Pathogenic Species

Abstract

Fungi produce a multitude of low-molecular-mass compounds known as secondary metabolites. Penicillium chrysogenum is known repositories of secondary metabolites. The interest in the secondary metabolites of Penicillium chrysogenum is due to their wide range of its biological activities. The present study was conducted to study the antibacterial activity of the crude extract of Penicillium chrysogenum isolated from the rhizospheric soil region of Mint. Samples were collected in a sterilized polythene bag from rhizospheric soil region of Mint. The samples were serially diluted and cultured on fungal media like potato dextrose agar and then purified and identified through colony morphology, microscopy and further molecular characterization through 18s RNA sequencing. The isolates were further cultured using potato dextrose broth media for production of biologically active secondary metabolites. The fungal extract was screened for anti-bacterial activities. The crude ethyl acetate extract of the Penicillium chrysogenum was used against five pathogenic bacterial species E. coli (22 mm), K. pneumonae (30mm), Pseudomonas (30mm) S.epidermitus (N/A) and Shigella (N/A). The ethyl acetate extract of Penecillium was tested against all these bacteria and the extract showed significant activity against all the bacteria. It is concluded from current study that Penicillium chrysogenum ethylacetate (EtoAc) extract has a great potential of inhibiting the growth of Pathogenic bacteria.