In Silico analysis of SOX2 genes mutations in Leukemia Patients through Ecosystem approach

Abstract

Human exposure to SO₂ and bisulfite (HSO^-₃) interact with SOX gene mutations which impact on AML and ALL patients is  divided into different parts. AML is a type of leukemia characterized by the rapid growth and accumulation of immature myeloid cells, which are a type of white blood cell. FLT3 is the mutation that occurs commonly in the AML which promotes the growth and proliferation of cells. The prognosis for AML depends on various factors, such as the age of the patient, specific genetic mutations, and overall health. The current study has been performed to identify the novel genetic mutations in the FLT3 protein which is the root cause of leukemia.

The PROVEAN software studies showed 95% single nucleotide polymorphism predicted as a neutral and 5% single nucleotide polymorphism predicted as a deleterious. The polyphen-2 software showed, 86.60 % single nucleotide polymorphism predicted as a benign, 6.60 % SNPs predicted as probably damaging and 6.60 % SNPs predicted as possibly damaging. I-Mutant 11 SNPs study shows that increase stability and 49 SNPs shows that decrease stability. According to SIFT software 48 % single nucleotide polymorphism predicted as a negative and 52 % single nucleotide polymorphism predicted as a positive. With the help of PhD-SNP software, 100 % single nucleotide polymorphism predicted as a deleterious and 0.0 % SNPs predicted as neutral.