Chemical Composition of the Ethyl Acetate Extract of Retama Raetam, HPLC-UV-MS Analysis.

Abstract

Since phytochemistry is mainly concerned with the separation, purification, and finally the structural determination of the separated compound, our contribution can be made in this field; Our work is part of the perspective of valuing and developing research on medicinal plants in Algeria and promoting traditional medicine; We chose the Retama Raetam plant from southeastern Algeria for the study.

   Through the results of the preliminary phytochemical examination, we confirmed the presence of flavonoids, so we extracted them by applying the most famous extraction methods (ethanol/water) (70/30); Where we got the highest yield in the butanol extract.

       The extracts obtained from the Retama Raetam plant were qualitatively analyzed by thin-layer chromatography, where 12 compounds were separated from ethyl acetate extract and 7 compounds from butanol extract.

   After that, we conducted a qualitative analytical study of the ethyl acetate extract by high-performance liquid chromatography attached to ultraviolet spectroscopy and HPLC-UV-MS mass spectrometry, through HPLC chromatogram analysis, it was found that there are 22 compounds. We proposed a chemical formula from the interpretation of the UV spectrum and the mass spectrum of the compound with Retention time Rt=16.37.

    Finally, phytotherapy constitutes an alternative role to the traditional pharmacy, and the need to find new molecules remains a public health priority.