Protective effects of Imedeen on in vitro fertilization potential in cyclophosphamide treated male mice

Abstract

The objective of the current study was to assess the protective effects of Imedeen (IME) in mice given cyclophosphamide (CP). 24 young, adult male mice were separated into 3 groups for this purpose. The second group received CP (12 mg/kg IP), while the control group merely received normal saline (0.2 mL per day, IP). For 35 days, the third group was given daily oral doses of 222 µg/kg IME in addition to CP. After that, the animals were put to sleep under anesthesia, and the sperm and epididymis were separated. Malondialdehyde (MDA) levels, DNA damage levels, and in vitro fertilty were all assessed. When compared to the control group, the number of sperms with damaged DNA and MDA levels in the CP-treated group increased significantly (p < 0.05). When compared to the CP group, the group receiving IME in addition to CP showed a decrease in DNA damage and MDA (p <0.05). Also, compared to the control group, the CP-treated group had significantly lower levels of in vitro fertilization and embryonic development, and there was a higher level of embryonic arrest (p <0.05). In vitro fertility and embryonic development were higher (p <0.05) and arrested embryos exhibited a decrease in the group that got IME in addition to CP. Imedeen may be able to lessen the negative consequences of CP, according to this study.