Isolation and Identification of Bacterial Pathogens Associated with Indwelling Devices and their Antimicrobial Susceptibility

Abstract

The current research project has been designed to evaluate the bacterial isolates in indwelling devices and their susceptibility pattern at Al-Syed Hospital Rawalpindi, a tertiary health care Hospital. The collection of microbial samples was performed from various indwelling devices at the hospital from indoor patients (hospital admitted). The bacterial samples collected were analyzed at Laboratory of the aforementioned hospital. After processing the samples were inoculated on Blood Agar, and MacConkey agar medium. The bacterial strains were allowed to get cultured for proper identification. Different identification techniques were followed for the cultured bacterial strains. After identifying, the pathogens were assessed for antibiotic sensitivity pattern by modified method of Kirby Bauer Disc diffusion technique. Similarly, suspected cases of hospital acquired infections were studied. The rate of indwelling devices infections were recorded as 23.4 %. The urinary (Foley’s Tips), respiratory (ETT Tips), central venous line (CVP Tips), DJ Tips and other (Suction Tube Tip, Drain Tube Tips, Perma Catheter Tip and NG Tube Tips) related infections were 27.4 %, 19.9%, 32.2%, 13.8% and 52.4% respectively. Escherichia Coli (16.2 %) and Enterococcus faecium 14.1 % were the most common isolate with maximum sensitivity to Colisten and Linezolid respectively. From the current investigational study it may be concluded that risk of hospital acquired infection was directly related to the placement of indwelling catheters and duration of the placement. It may also be inferred that the indwelling devices in the hospitals may be soft corner for gram positive, gram negative, aerobic, inaerobic, facultative aerobic and facultative in-aerobic bacterial strains.