Analysis of active biochemical compounds of Pstroselinum crispum and Apium graveolens aqueous extracts by Gas chromatography-Mass Spectrometry (GC-MS) and their antibacterial activity.
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Abstract
The present study aimed to evaluate the antibacterial activity of plant extracts and their effect on isolated human pathogen bacteria and comparison of the effect of the active compounds of Petroselinum crispim extract and Apium graveolens extract, The results of GC-Mass of hot aqueous extract of Petroselinum crispim showed many active compounds through 10 peaks, but the highest area (7.37) of active compounds found in peak No.5 as (Trolamine and Benzofuran). While the results of hot aqueous extract of Apium graveolens showed the highest area (3.83) of active compounds found in peak No.1 as (Pentanoic acid).
The study was conducted on 100 samples were collected between March 2022 and May 2022, where the number of positive samples reached 83, while the number of negative samples reached 17, by taking urine samples from various patients in Ibn Al-baladi Hospital in Baghdad. Two types of bacteria were isolated: Escherichia coli, which appeared in 46 samples and 37 samples from Staphylococcus bacteria, all bacterial isolates were identified.
The results showed that the hot aqueous extract of Petroselinum crispum give better inhibition zone in concentration No.1 (10mm) than in concentration No.2 (7mm) on staphylococcus aureus. The hot aqueous extract of Petroselinum crispum give better inhibition zone in concentration No.1 (7.33mm) than in concentration No.2 (7mm) on Escherichia coli.
The hot aqueous extract of Apium graveolens give better inhibition zone in concentration No.1 (9.66mm) than in concentration No.2 (7.66mm) on staphylococcus aureus. The hot aqueous extract of Apium graveolens give the same inhibition zone in concentration No.1 and concentration No.2 (8.33mm) on Escherichia coli.