Investigating the regulatory role of miR-663a in the tissue of patients with glioblastoma multiforme cancer compared to healthy tissue

Abstract

Introduction

A new group of regulatory biomarkers, which are about 22 to 24 nucleotides long, are micro RNAs. Investigating the dysregulation of miRNA regulation in cancers has recently become a focus of research. These small structures can act as oncogenes and tumor suppressors. In the present study, the relationship between the expression level of miR-663a in the tissues of patients with glioblastoma multiforme and that of healthy individuals was investigated. Moreover, the correlation between the expression level of miR-663a and age, gender and tumor grade was done.

Materials and Methods

In this study, which was conducted on 50 patients with glioblastoma multiforme cancer and 50 patients as a control group, the genomic content of miR-663a was measured using Real-Time PCR technique. The biomarker value of miR-663a was evaluated using the ROC curve.

Results

The research results show that the expression level of miR-663a is higher in patient samples compared to healthy ones (Pv=0.0001). The increase in the concentration of miR-663a in serum samples in patients has a significant relationship with the final grade of disease progression of grade I and IV (Pv=0.0173). The curve analysis and the determination of the 6.145 cut-off level was found that miR-663a with the specificity of 0.78 and sensitivity of 1 can detect glioblastoma multiforme malignancy in the patients’ serum samples compared to healthy individuals.

Discussion and Conclusions

In this study, the possible oncogenic role of miR-663a has been identified and it is possible to check the progress of glioblastoma cancer in the final stages by measuring its level in the serum of patients.