Analysis of active biochemical compounds of Pstroselinum crispum and Apium graveolens alcoholic extracts by Gas chromatography-Mass Spectrometry (GC-MS)and their antibacterial activity

Abstract

The present study aimed to evaluate the antibacterial activity of plant extracts and their effect on isolated human pathogen bacteria and comparison of the effect of the active compounds of Petroselinum crispim extract and Apium graveolens extract, The results of GC-Mass of hot alcoholic extract of Petroselinum crispim showed many active compounds through 10 peaks, but the highest area (8.47) of active compounds found in peak No.7 as (3,7-dimethyl-6-octenyl ester, Butanoic acid, Bicyclo[3.1.1]heptane and 2,6,6-trimethyl). While the results of hot alcoholic extract of Apium graveolens showed the highest area (8.79) of active compounds found in peak No.6 as (Fumaric acid, Propanoic acid, 2-methyl-4-methylphenyl ester and Benzene).                                                                                                               

The study was conducted on 100 samples were collected between March 2022 and May 2022, where the number of positive samples reached 83, while the number of negative samples reached 17, by taking urine samples from various patients in Ibn Al-baladi Hospital in Baghdad. Two types of bacteria were isolated: Escherichia coli, which appeared in 46 samples, 37 samples from Staphylococcus bacteria, all bacterial isolates were identified.                            

The results showed that the hot alcoholic extract of Petroselinum crispum give better inhibition zone in concentration No.1 (9.66mm) than in concentration No.2 (8.33mm) on staphylococcus aureus. While the hot alcoholic extract of Petroselinum crispum give better inhibition zone in concentration No.2 (13.33mm) than in concentration No.1 (12mm) on Escherichia coli.                  

The hot alcoholic extract of Apium graveolens give better inhibition zone in concentration No.2 (10mm) than in concentration No.1 (9.66mm) on staphylococcus aureus. The hot alcoholic extract of Apium graveolens give better inhibition zone in concentration No.2 (13mm) than in concentration No.1 (11.66mm) on Escherichia coli.