Novel criteria of Egyptian population using Y-chromosome STRs by Yfiler Plus® System (A: Allele Frequency)

Abstract

Background: The male-specific part of the human Y chromosome is globally used in forensic DNA analysis, identification of males for criminal justice purpose and population genetics, especially in situations where standard autosomal DNA profiling is not adequately informative. The aim of this study was to develop an allelic frequency database for the Egyptian population in order to obtain population data of 27 Y-STRs in order to appraise the resolution power of these loci in differentiating

Closely- related male individuals. 

Materials and methods: In this study, we used the Yfiler® Plus kit, which includes 7 rapidly mutating loci (RM Y-STRs) and 20 standard Y-STR, to analyse 200 unrelated males coming from all the Egyptian governorates. For Y-STR analysis, 200 male buccal swap samples were used in this study, The recently introduced 6-dye Y filer Plus multiplex which includes 27 Y-STR loci (DYS576, DYS389I, DYS635, DYS389II, DYS627, DYS460, DYS458, DYS19, YGATAH4, DYS448, DYS391, DYS456, DYS390, DYS438, DYS392, DYS518, DYS570, DYS437, DYS385 a/b, DYS449, DYS393, DYS439, DYS481, DYF387S1a/b and DYS533) has been used. These RM Y-STRs are useful for discriminating between closely related and unrelated males.

Results: Five thousand and four hundred alleles were detected at the 27 Y-STR loci in 200 samples. Alleles frequency ranged from 1 to 159 and the highest allele frequency registered by allele 14 was 159 at locus DYS437 recording a percentage of 79.5% inside this locus followed by allele 11 with 151 allele frequency recording a percentage of 75.5% at locus DYS392. Also, the highest predominant allele frequency regestered was 0.80 for the predominant allele 17 at locus DYS570, while the lowest frequency was 0.22 for the predominant alleles 18 and 25 at loci DYS385 B and DYS481  respectively.

Conclusion: The present study established the genetic information obtained using the Yfiler Plus® system for the Egyptian  population and also created a database of 27 Y- STR markers in this population on the basis of allele dominance and frequencies.