In-Vitro Assessment of Antimicrobial and Cytotoxic Activities of Wulfenia Amherstiana against the Human Breast Adenocarcinoma (MCF-7) and Human Hepatocellular Liver Carcinoma (Hepg-2) Cell Lines

Abstract

To investigate the cytotoxic activity against human cancer cell lines and antimicrobial (antibacterial and antifungal) potential of Wulfenia amherstiana or Wulfeniopsis amherstiana by preparing its ethanolic extract and n-hexane, chloroform, n-butanol and ethyl acetate fractions. Antimicrobial activities were determined through agar well diffusion method. MTT assay was used to assess the cytotoxic activity against human breast adenocarcinoma (MCF-7) and human hepatocellular carcinoma (HepG-2) cell lines. Antibacterial assays revealed that chloroform fraction showed maximum efficacy against gram positive bacteria Staphylococcus aureus NCTC 6571 (95.2% inhibition) and against Pseudomonas aeruginosa ATCC 10145 (96.2% inhibition). Both Chloroform (90% Inhibition) and n-hexane (80 %Inhibition) fractions were found most efficacious against Aspergillus flavus ATCC 32611. The MTT assay revealed considerable cytotoxicities of plant ethanolic extract (IC50 = 50 µg/ml, 100 µg/ml against MCF-7 and HepG-2 cell lines, respectively), and chloroform (IC50 = 100 µg/ml and 150 µg/ml against MCF-7 and HepG-2 cell lines) and ethyl acetate fractions (IC50 = 150 µg/ml and 200 µg/ml against MCF-7 and HepG-2 cell lines). The n-butanol and n-hexane fractions were comparatively less cytotoxic. The present study concluded that the W. amherstiana possesses significant antibacterial, antifungal, and cytotoxic activities. The ethanolic extract, its chloroform and ethyl acetate fractions were found moderately cytotoxic proving the plant’s anticancer activity.