PGPR: A microbial Resource to Promote the Growth of Legumes and to Enhance their symbiotic potential, case of pea (Pisum sativum)

Abstract

Beneficial bacteria, known as plant growth promoting rhizobacteria (PGPR), can improve legume growth and symbiotic nitrogen fixation by colonizing plant roots. In pea agriculture, PGPR can enhance yield, improve stress tolerance, and lessen the need for chemical fertilizers by boosting nutrient uptake, inhibiting infections, and increasing nodule formation. In this work, examining the effects on the growth of Pisum sativum, or pea plants, this study isolates, identifies, and evaluates Plant Growth-Promoting Rhizobacteria (PGPR) from rhizospheric soil in the Tissemsilt region of Algeria. A total of eight bacterial species (B2, B4, B5, S3, S4, S5, S6, S7) were identified and characterized using morphological, biochemical, and API 20 NE methodologies. We looked at antibiotic resistance patterns and tested how well different strains could stimulate plant growth. Following the introduction of the bacterial strains to the pea seeds, the impact on fresh and dry weight as well as the number of root nodule formations was assessed. In order to identify any correlations between bacterial abundance and soil fertility, a physical-chemical analysis of the soil was conducted. The results demonstrated that certain bacterial strains, such as Pseudomonas luteola and Pseudomonas fluorescens, significantly enhanced nodulation and plant growth, implying that these microbes could serve as valuable biofertilizers for environmentally conscious farming.